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Human TIGIT Protein, hFc Tag

Recombinant human TIGIT Protein with C-terminal human Fc tag


Cat.#:  11225

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11225
Product Name:   Human TIGIT Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   TIGIT;SIG9;STM3
Target:    TIGIT
Description:    Recombinant human TIGIT Protein with C-terminal human Fc tag
Background:     This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity, this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 39.13 kDa after removal of the signal peptide.The apparent molecular mass of TIGIT-hFc is approximately 40-53 kDa due to glycosylation.
Molecular Characterization:    TIGIT(Met22-Pro141) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human TIGIT Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD155, mFc-His tagged protein 11155 can bind Human TIGIT, hFc tagged protein (11225) in a linear range of 1.95-125 ng/ml.

Figure 3.  Flow cytometry analysis with 1µg/ml Human TIGIT Protein, hFc tag (11225) on Expi293 cells transfected with human CD112 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).