$495.00
| Anti-Rab11GTP Mouse Monoclonal Antibody |
| Cat. #: 26919 |
| Size: 30 µL |
| Gene Symbol: RAB11 |
| Description: Anti-Rab11-GTP Mouse Monoclonal Antibody |
| Background: Small GTPases of the Rab-family are crucial elements of the machinery that controls membrane traffic. Rab11 has been shown to control traffic through the recycling endosome. |
| Immunogen: Recombinant full length protein of active Rab11 |
| Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Rab11 GTPase) |
| Published Applications: IP, IHC and IF – Click for Details |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG2b |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-Rab11-GTP monoclonal antibody recognizes active Rab11 from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
Rab11 Activation Assay.
Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-Rab11-GTP monoclonal antibody (Cat. No. 26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21157). |
