$495.00
| Anti-Rab4GTP Monoclonal Antibody |
| Cat. #: 26921 |
| Size: 30 µL |
| Gene Symbol: RAB4 |
| Description: Anti-Rab4-GTP Monoclonal Antibody |
| Background: Rab-4 belongs to the small GTPase Rab family. It interacts with RAB11FIP1, RABEP1, ZFYVE20, RUFY1, SGSM1, SGSM2 and SGSM3. It participates in protein transport and in vesicular traffic. |
| Immunogen: Recombinant full length protein of active Rab4A. |
| Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Rab4 GTPase) |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-Rab4-GTP monoclonal antibody recognizes active Rab4 from vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
Rab4A Activation Assay.
Purified GST-tagged Rab4A proteins (Cat. #10130) were immunoprecipitated with the anti-Rab4-GTP monoclonal antibody (Cat. No. 26921) after treated with GDP (lane 1) or GTPγ S (lane 2), and was blotted with anti-Rab4A polyclonal antibody (Cat. No. 21067). Input control is shown in the bottom panel. |
