Cat.#: 28051 |
Product Name: Anti-CD40L (Ruplizumab) Monoclonal Antibody |
Synonyms: CD40LG;D154;D40L;IGM1;GM;MD3;-BAM;NFSF5;RAP;p39 |
Description: Anti-CD40L (ruplizumab ) Monoclonal Antibody |
Background: Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
Applications: ELISA; Flow Cyt |
Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
Host Species: Humanized |
Isotype: IgG1 |
Purification: Purified from cell culture supernatant by affinity chromatography |
Species Reactivity: Human CD40 Ligand |
Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
 Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD40L, hFc tagged protein (11241) can bind Anti-CD40L Neutralizing antibody (28051) in a linear range of 0.64-400 ng/ml. In order to specifically detect 28051, mouse anti-human Fab-specific antibody was used as detection antibody. |  Figure 2. HEK293 cell line transfected with irrelevant protein (B) and human CD40L (A) were surface stained with Anti-CD40L mAb 1 µg/ml (ruplizumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody. |
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