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SARS-CoV-2 (Delta) S protein RBD, His Tag

Recombinant SARS-CoV-2 RBD(L452R,T478K) protein with C-terminal 6XHis tag


Cat.#:  11443

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11443
Product Name:   SARS-CoV-2 (Delta) S Protein RBD
Size :    10 µg, 50 µg and 100 µg
Synonyms:   SARS-CoV-2 B.1.617.2 (Delta) Spike RBD Protein
Target:    S protein RBD
Description:    “Recombinant SARS-CoV-2 RBD(L452R
Background:     SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 26.0 kDa after removal of the signal peptide. The apparent molecular mass of RBD(L452R,T478K)-His is approximately 25-35 kDa due to glycosylation.
Molecular Characterization:    RBD(L452R,T478K)(Ser19-Glu351) 6×His tag
Purity:    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. SARS-CoV-2 RBD(L452R,T478K) Protein, His Tag on SDS-PAGE under reducing condition.