Email: info@neweastbio.com   |  Call: 6109452007

SARS-CoV-2 RBD (DM35) Rabbit mAb

Anti-SARS-CoV-2 RBD (DM35) Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  28324

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28324
Product Name:   Anti-SARS-CoV-2 RBD(DM35) Rabbit Monoclonal Antibody
Synonyms:   SARS-CoV-2 RBD
Description:    Anti-SARS-CoV-2 RBD antibody(DM35) Rabbit Monoclonal Antibody
Background:    SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits; S1 and S2. S1 mainly contains a receptor binding domain (RBD); which accounts for recognizing the cell surface receptor; ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    SARS-CoV-2 S protein RBD
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. Elisa plate pre-coated by 2 µg/ml (100µl/well) SARS-CoV-2 RBD protein can bind Rabbit Anti-SARS-CoV-2 RBD monoclonal antibody ( clone:DM35) in a linear range of 0.19-200 ng/ml.

Figure 2. Competition flow cytometry assay demonstrating Rabbit anti-RBD monoclonal antibody ( clone: DM35) blockade of SARS-CoV-2 (COVID-19) S protein RBD (1µg/ml, [getskuurl sku=11301 binding to Expi 293 cell line transfected with human ACE2. IC50=1013ng/ml. The Y-axis represents the geometric mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
          Publications