| Cat.#: 11124 |
| Product Name: Human TLR5 Full Length Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: MELIOS; SLE1; SLEB1; TIL3 |
| Target: TLR5 |
| UNIPROT ID: O60602 |
| Description: Human TLR5 full length protein-synthetic nanodisc |
| Background: Toll-like receptor (TLR) family plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease. |
| Species/Host: HEK293 |
| Molecular Weight: The human full length TLR5 protein has a MW of 97.8 kDa |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure1. Elisa plates were pre-coated with Flag Tag TLR5-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TLR5-Nanodisc is 4.191ng/ml. | 
Figure2. Human TLR5-Nanodisc, Flag Tag on SDS-PAGE |
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