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Human ACE2 Protein, His Tag

Recombinant Human ACE2 Protein with N-terminal 6XHis tag
5/5

$445.00

Cat.#:  11296

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11296
Product Name:   Human ACE2 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   ACE-2;CEH;CE2
Target:    ACE2
UNIPROT ID:    Q9BYF1
Description:    Recombinant Human ACE2 Protein with N-terminal 6xHis tag
Background:     The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 84.4 kDa after removal of the signal peptide. The apparent molecular mass of His-ACE2 is approximately 100-130 kDa due to glycosylation.
Molecular Characterization:    6×His ACE2(Gln18-Ser740)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human ACE2 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human ACE2, His Tagged protein(11296)can bind S-RBD, hFc tagged protein 11295 in a linear range of 0.128-80.0 ng/ml.
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