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Human GUCY2C Protein, His Tag

Recombinant human GUCY2C protein with C-terminal 6XHis tag


Cat.#:  11252

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11252
Product Name:   Human GUCY2C Protein
Size :    10 µg, 50 µg and 100 µg
Target:    GUCY2C
UNIPROT ID:    P25092
Description:    Recombinant human GUCY2C protein with C-terminal 6xHis tag
Background:     This gene encodes a transmembrane protein that functions as a receptor for endogenous peptides guanylin and uroguanylin, and the heat-stable E. coli enterotoxin. The encoded protein activates the cystic fibrosis transmembrane conductance regulator. Mutations in this gene are associated with familial diarrhea (autosomal dominant) and meconium ileus (autosomal recessive). [provided by RefSeq, Nov 2016]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 46.8 kDa after removal of the signal peptide. The apparent molecular mass of GUCY2C-His is approximately 55-130 kDa due to glycosylation.
Molecular Characterization:    GUCY2C(Ser24-Gln430) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human GUCY2C Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human GUCY2C protein, His Tag (11252) can bind Anti-GUCY2C Neutralizing antibody 28073 in a linear range of 3.2-400 ng/mL.