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Human ICOS Protein, mFc-His tag

Recombinant human ICOS protein with C-terminal mouse Fc and 6XHis tag


Cat.#:  11154

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11154
Product Name:   Human ICOS Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   ICOS;CD278;AILIM;Inducible T-cell costimulator
Target:    ICOS
Description:    Recombinant human ICOS protein with C-terminal mouse Fc and 6xHis tag
Background:     The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 41.1 kDa after removal of the signal peptide.
Molecular Characterization:    ICOS(Glu21-Lys140) mFc(Pro99-Lys330) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human ICOS Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human ICOS, mFc-His tagged protein (11154) can bind Anti-ICOS Neutralizing antibody )]in a linear range of 0.24-31.25ng/ml.