| Cat.#: 11444 |
| Product Name: Human NEFL (89-400) Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: CMT1F;CMT2E;CMTDIG;NF-L;NF68;NFL;PPP1R110 |
| Target: NEFL |
| UNIPROT ID: P07196 |
| Description: Recombinant human NEFL(89-400) protein with N-terminal 6xHis tag |
| Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 36.9 kDa after removal of the signal peptide. The apparent molecular mass of His-NEFL(89-400) is approximately 35-55 kDa due to glycosylation. |
| Molecular Characterization: 6×His tag NEFL(Gln89-Leu400) |
| Purity: The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human NEFL(89-400) Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human NEFL (89-400) Protein, His Tag (11444) can bind Anti-NEFL(89-400) antibody(DM199), Rabbit mAb 28542 in a linear range of 0.128-80 ng/mL. |
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