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Anti-NOTCH1(M2011R) Mouse Monoclonal Antibody


Cat.#:  26444

   Size:   100 μl

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            Product Description          
Cat. #:  26444
Size:  100 µL
Gene Symbol:  NOTCH1, AOS5, AOVD1,TAN1, hN1
Description:   Anti-NOTCH1(M2011R) Mouse Monoclonal Antibody
Background:   NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2.
Immunogen:   A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:    ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes M2011R mutant, but not wild type NOTCH1 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant NOTCH1(M2011R) and wild type proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild type protein (lane2) were blotted with Anti-NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
Immunofluorescence:   Immunofluorescence of cells expressing NOTCH1 proteins with Anti-NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP-NOTCH1 (WT) plasmid (left column) or pCDNA3-GFP-NOTCH1(M2011R) plasmid (right column), then fixed and stained with Anti-NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
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