$495.00
| Cat. #: 26444 |
| Size: 100 µL |
| Gene Symbol: NOTCH1, AOS5, AOVD1,TAN1, hN1 |
| Description: Anti-NOTCH1(M2011R) Mouse Monoclonal Antibody |
| Background: NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2. |
| Immunogen: A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin. |
| Applications: ELISA, WB, IF, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes M2011R mutant, but not wild type NOTCH1 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Western blot:
Western blot analysis of recombinant NOTCH1(M2011R) and wild type proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild type protein (lane2) were blotted with Anti-NOTCH1(M2011R) monoclonal antibody (Cat. #26444). |
Immunofluorescence: 
Immunofluorescence of cells expressing NOTCH1 proteins with Anti-NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP-NOTCH1 (WT) plasmid (left column) or pCDNA3-GFP-NOTCH1(M2011R) plasmid (right column), then fixed and stained with Anti-NOTCH1(M2011R) monoclonal antibody (Cat. #26444). |
