$495.00
| Anti-RalGTP Mouse Monoclonal Antibody |
| Cat. #: 26913 |
| Size: 30 µL |
| Gene Symbol: Ral |
| Description: Anti-Ral-GTP Mouse Monoclonal Antibody |
| Background: The Ras-like small G proteins, RalA and RalB, are important components of Ras signaling pathways, implicated in the initiation and maintenance of tumorigenic transformation, as well as vesicle transport, apoptosis, transcription, cell migration, and cell proliferation. |
| Immunogen: Recombinant full length protein of active RalB. |
| Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Ral GTPase) |
| Published Applications: IP, IHC and IF – Click for Details |
| Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Anti-Ral-GTP monoclonal antibody recognizes active RalA and RalB of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western blot:
Ral activation assay.
Purified RalB proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Ral-GTP monoclonal antibody (Cat. No. 26913). Immunoblot was with an anti-RalB rabbit polyclonal antibody (Cat. No. 21033). |
| 1. Exocyst protein subnetworks integrate Hippo and mTOR signaling to promote virus detection and cancer Cell Rep. 20213 PMID: 4348154 |
| 2. RalGTPases contribute to Schwann cell repair after nerve injury via regulation of process formation J Cell Biol. 2019 PMID: 31201266 |
